Journal: Cardiovascular Research

Article Title: STAT3-miR-17/20 signalling axis plays a critical role in attenuating myocardial infarction following rapamycin treatment in diabetic mice

doi: 10.1093/cvr/cvz315

Figure Lengend Snippet: Role of STAT3 in hearts of diabetic mice following rapamycin treatment. (A) Representative immunoblots for p-STAT3, total STAT3, and GAPDH expression in whole heart of C57 and db/db mice following 28 days of RAPA treatment. Densitometry analysis of immunoblots for the ratio of p-STAT3/STAT3 (*P < 0.05 vs. others) and the ratio of STAT3/GAPDH (*P < 0.05 vs. C57-Control; n = 3). (B) Myocardial infarct size following ischaemia/reperfusion in STAT3-deficient diabetic mice. High-fat diet (HFD)-fed WT and STAT3-deficient mice were treated with RAPA for 28 days prior to evaluation of I/R injury. Upper panel: representative images of heart sections following TTC staining (Scale indicates 5 mm). Lower panels: quantitative data of infarct size following I/R injury (*P < 0.001 vs. other) and rate-force product (*P < 0.01 vs. other; n = 5). (C) Isolated cardiomyocyte necrosis was determined by trypan blue staining following 40 min SI and 1 h RO (n = 4–8). (D) Representative pictures of TUNEL staining (scale indicates 100 µm) and quantitative data of cardiomyocyte apoptosis following 40 min SI and 18 h RO (n = 4; *P < 0.001 vs. controls, αP < 0.001 vs. both SI/RO and βP < 0.05 vs. WT-SI/RO). Statistics: one-way ANOVA.

Article Snippet: 23 Apoptosis was determined after 40 min SI and 18 h of RO using TUNEL staining kit (BD Biosciences, San Jose, CA, USA) as previously reported.

Techniques: Western Blot, Expressing, Staining, Isolation, TUNEL Assay

Journal: Theranostics

Article Title: Iron Oxide Nanoparticles Promote Cx43-Overexpression of Mesenchymal Stem Cells for Efficient Suicide Gene Therapy during Glioma Treatment

doi: 10.7150/thno.60160

Figure Lengend Snippet: In vivo therapeutic gains of using MFIONs-harbouring MSCs for suicide gene therapy against glioma. A) Schematic illustration to show the details of the therapeutic study. B) Survival curves of glioma rats after the different treatments. The differences between groups were compared with PBS group. C) Images of fixed brain samples and Nissl-stained brain sections. D) The areas of the gliomas were calculated with ImageJ according to the Nissl staining results. E) Pathological images of the cerebral sections (including H&E and TUNEL immunohistochemical staining). B) N.S.: no significant difference , **p < 0.01, compared to PBS group, based on Logrank. D) N.S.: no significant difference, **p < 0.01, based on one-way ANOVA. n = 5 for B). n = 3 for D). Data are means ± SD.

Article Snippet: A TUNEL immunohistochemical staining kit (ZK8004, ZSGB-BIO, Beijing, China) was performed to stain the brain samples following the manufacturer's protocol.

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemical staining